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目的:观察腰椎“椎骨错缝”模型大鼠痛阈变化。方法:采用椎体外部连接固定系统(ELFS)诱导“椎骨错缝”大鼠模型。将160只350~450 g雄性SD大鼠随机分为正常对照组、假手术组、模型对照组和实验组。实验组通过外科手术将ELFS的棘突附着部件(SAU)与大鼠L4、L5和L6棘突相连接,再使用ELFS的外部连接部件将SAU连接起来;模型对照组仅植入SAU,不安装外部连接部件;假手术组仅进行手术干预,不植入任何部件;正常对照组不进行任何干预。再将各组按固定时间分为1、2、4、8和12周5个亚组。对每组中8只大鼠进行机械痛阈和热痛阈检测。结果:实验组的机械痛阈在4周及8周时与对照组出现统计学差异(P<0.05);实验组各时间点间机械痛阈随时间延长呈逐渐下降的趋势。实验组的热痛阈在2周时间点时与对照组出现统计学差异(P<0.05),这种差异一直持续到8周;实验组各时间点间热痛阈值呈逐渐下降的趋势,4周时热痛阈和1、2周时间点相比出现显著差异(P<0.05),并持续到8周。结论:“椎骨错缝”模型大鼠现伴随着生物力学的改变,其行为学也发生着相似的变化。
Objective: To observe the changes of pain threshold in lumbar spondylolisthesis rats. Methods: Sprague-Dawley rat model was induced by ELFS. 160 male SD rats weighing 350-450 g were randomly divided into normal control group, sham operation group, model control group and experimental group. In the experimental group, the ELFS spinous process attachment (SAU) was surgically connected to the rat L4, L5 and L6 spinous processes, and then the ELU was used to connect the SAU. The model control group was only implanted with SAU, External connection components; sham operation group only surgical intervention, no components were implanted; normal control group without any intervention. The groups were divided into five subgroups of 1, 2, 4, 8 and 12 weeks according to a fixed time. Eight rats in each group were subjected to mechanical and thermal pain thresholds. Results: The mechanical pain threshold of the experimental group was significantly different from that of the control group at 4 weeks and 8 weeks (P <0.05). The mechanical pain threshold of the experimental group decreased gradually with time prolonging. The pain threshold of the experimental group was statistically different from that of the control group at 2 weeks (P <0.05), and this difference lasted for 8 weeks. The pain threshold of the experimental group showed a gradual decrease 4 At week 1, there was a significant difference (P <0.05) in the thermal pain threshold compared with that at 1 and 2 weeks, which lasted for 8 weeks. CONCLUSIONS: The “Spina bifida” model rat is now accompanied by biomechanical changes and its behavior changes similarly.