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目的 :探讨建立免疫介导小鼠再障模型中的最适照射剂量。方法 :采用不同剂量的 [60 Co γ]射线对BALB/c小鼠进行全身照射 ,并输入DBA/2小鼠的胸腺、淋巴结混合细胞 ,于照射并输入细胞后第 14d处死实验小鼠观察股骨骨髓增生程度及病理改变 ,以确定诱导再障发生的情况。结果 :6 0Gy、5 5Gy和 5 0Gy各照射剂量再障诱导组的再障发生率分别为 85 7%、84 6 %和 2 2 2 %。各实验组小鼠第 14d存活率分别为 5 7 1%、10 0 %和10 0 %。结论 :本实验条件下 ,5 5Gy的照射剂量对建立免疫介导小鼠再障模型较为合适。
OBJECTIVE: To investigate the optimal radiation dose in immune-mediated aplastic anemia mice model. Methods: BALB / c mice were irradiated with different doses of [60 Co γ] radiation, and were injected into thymus and lymph node mixed DBA / 2 mice. The mice were sacrificed on the 14th day after irradiation and input into the cells. Myeloproliferation and pathological changes to determine the incidence of aplastic anemia. Results: The incidences of aplastic anemia in the groups of 6 Gy, 5 5 Gy and 50 Gy irradiation were 85.7%, 84.6% and 22.2% respectively. The 14th day survival rate of mice in each experimental group was 57.1%, 100% and 100% respectively. Conclusion: Under the experimental conditions, a radiation dose of 5 5 Gy is more suitable for the establishment of immune-mediated aplastic anemia in mice.