为提供急性心肌梗塞(AMI)早期血清学诊断法,用改进后的 Sch βler 法提取狗肌凝蛋白轻链(CM-LC),免疫兔获得抗血清。氯胺 T 法标记抗原,建立血清 CM-LC RIA。结果:该方法标准曲线测量范围1～100μg/L,批内、批间 CV 分别是3.2%和9.1%,平均回收率95.3%。正常人30例血清 CM-LC 为3.5±1.5μg/L,20例 AMI 患者血清 CM-LC 为20.2±8.6μg/L,两组间差异非常显著。提示血清 CM-LC RIA 可用于 AMI 早期诊断。 To provide an early serological diagnosis of acute myocardial infarction (AMI), a modified Schβler method was used to extract dog myosin light chain (CM-LC) and immunized rabbits to obtain antiserum. Chloramine T method labeled antigen, the establishment of serum CM-LC RIA. Results: The standard curve range of this method was 1 ~ 100μg / L, intra-assay and inter-assay CV were 3.2% and 9.1%, respectively. The average recovery was 95.3%. The serum CM-LC of 30 normal individuals was 3.5 ± 1.5μg / L, and the serum CM-LC of 20 AMI patients was 20.2 ± 8.6μg / L. There was a significant difference between the two groups. Prompt serum CM-LC RIA can be used for early diagnosis of AMI.