Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arth

来源 :Chinese Journal of Oceanology and Limnology | 被引量 : 0次 | 上传用户:wanyu123456
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
We adopted the response surface methodology using single factor and orthogonal experiments to optimize four types of antimicrobial agents that could inhibit biofilm formation by Streptococcus mutans, which is commonly found in the human oral cavity and causes tooth decay. The objective was to improve the function of marine Arthrobacter oxydans KQ11 dextranase mouthwash(designed and developed by our laboratory). The experiment was conducted in a three-level, four-variable central composite design to determine the best combination of Zn SO_4, lysozyme, citric acid and chitosan. The optimized antibacterial agents were 2.16 g/L Zn SO_4, 14 g/L lysozyme, 4.5 g/L citric acid and 5 g/L chitosan. The biofilm formation inhibition reached 84.49%. In addition, microscopic observation of the biofilm was performed using scanning electron microscopy and confocal laser scanning microscopy. The optimized formula was tested in marine dextranase Arthrobacter oxydans KQ11 mouthwash and enhanced the inhibition of S. mutans. This work may be promoted for the design and development of future marine dextranase oral care products. We adopted the response surface methodology using single factor and orthogonal experiments to optimize four types of antimicrobial agents that could inhibit biofilm formation by Streptococcus mutans, which is commonly found in the human oral cavity and causes tooth decay. The objective was to improve the function of marine Arthrobacter oxydans KQ11 dextranase mouthwash (designed and developed by our laboratory). The experiment was conducted in a three-level, four-variable central composite design to determine the best combination of Zn SO 4, lysozyme, citric acid and chitosan. The optimized antibacterial The agents were 2.16 g / L Zn SO4, 14 g / L lysozyme, 4.5 g / L citric acid and 5 g / L chitosan. The biofilm formation inhibition reached 84.49%. In addition, microscopic observation of the biofilm was performed using scanning electron microscopy and confocal laser scanning microscopy. The optimized formula was tested in marine dextranase Arthrobacter oxydans KQ11 mouthwash and enhanced the inhib ition of S. mutans. This work may be promoted for the design and development of future marine dextranase oral care products.
其他文献
给认证一个理由先 “我用那么多的时间去接受培训,以便获得认证证书,就是希望自己以后能够有更多的选择和更大的发展空间。就算近期效果很难有清晰的凸现,但我确信通过考试
多孔硅可见光发射现象是近一两年半导体物理学界的重大发现。1992年初,美国物理学会从1991年凝聚态物理和材料物理的80多项进展中选出七大进展,多孔硅可见光发射就是其中之
这天.信子来到一家百货商场。一进门.她的脚步就不由自主地变得轻飘飘的。这里时髦的服装,进口的百货,真是琳琅满目,看得人眼花缭乱。
2007年4月20日,上海新国际博览中心,是国内主流重卡企业的练兵场。随着各家卡车制造商在上海国际车展上将自己配置最高、品质最好的产品“整装列队”,“尽情”向前来参观的观
人类一直追求人工智能能达到人脑,甚至超越人类大脑的水平,于是电影里有了阿童木,有了AI机器人,但这些只是想象,因为人类远没有做好接纳具有人工智能机器人的准备,或者说,人类对于智能机器人的期待里总是伴随着某种恐惧,这种纠结于完美智能与未知危险的复杂情绪,常常成为机器人科幻电影的核心主题。西班牙科幻电影《伊娃》里,人工智能机器人作为“危险”的自由机器人被法律禁止,但总有例外而让一段悲情故事续写出新的情
培育母树林的目的在于获得量多质优的种子,而各种培育措施应用是否得当,对此却有很大影响。因此,根据落叶松的结实习性,合理采取技术措施是十分必要的。修枝,在果树园艺上已
当前的高中英语学习中,学生的弱点很多,尤其在阅读能力和写作能力上普遍不如人意,在教学中英语的阅读和写作是相关联的,阅读能提高写作能力,反过来写作也能提高学生的阅读能
多媒体应用于英语课堂教学,对于课堂教学的优化、提高课堂教学效率,对于培养学生的综合能力,有着十分重要的作用。在英语教学中,要使学生真正处于主体地位,激励其积极参与到
利用Maxwell电磁理论,推导电磁波在一维光子晶体中传播的传输矩阵通式,进一步讨论各类介质一维光子晶体中传输矩阵通式的适用条件,并加以实例对相应传输矩阵进行验证计算,为
教育写作,是教师介入教育生活的重要途径,是教师专业成长的必经之路.华东师大叶澜教授曾说:“一个人写一辈子教案不一定能成为一个名师,但是,如果写三年反思就可能成为名师.