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一、概况:本世纪初植物学家在研究如何分离色素时,发现将植物色素的有机溶液,流过装有粉状填充剂的玻璃柱后,植物色素中的各种成分由于被吸附能力的差异而浓集在管柱的不同高度,形成不同的色谱带。于是一种行之有效的分离方法一色谱法(也称层析法)就这样诞生了。此后经过许多科学家多年的改进和发展,又相继建立了纸上色谱(PC),薄层色谱(TLC),气相色谱(GC)等。自70年代始,由于色谱柱应用了颗粒小(5～10微米)的填充剂,配用了高压输液
First, an overview: The beginning of this century botanists in the study of how to separate the pigment, the plant pigment found in the organic solution flow through the glass filled with powdered filler, the various components of plant pigments due to be adsorbed Differences and concentration in the column at different heights, the formation of different bands. So a proven separation method of chromatography (also known as chromatography) was born. Since then, many scientists have made improvements and developments over the years and have successively established paper chromatography (PC), thin layer chromatography (TLC) and gas chromatography (GC). Since the 1970s, due to the small particle size of the column (5 to 10 microns), a high-pressure infusion