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目的研究影响复方抗癌散中有效抗癌成分提取工艺的主要因素,优化复方抗癌散的提取工艺。方法以正交试验法设计实验,以高效液相色谱法进行含量测定,最终以得膏率和样品中熊果酸的含量为指标优选复方抗癌散的提取工艺。结果优选所得复方抗癌散的提取工艺为溶剂用量12倍,提取时间1.5h,乙醇水溶液浓度80%。结论优化提取工艺条件下的复方抗癌散的干膏和熊果酸含量均较高,并适宜于工业生产。
Objective To study the main factors influencing the extraction process of effective anti-cancer components in Compound Anticancer Powder and optimize the extraction process of Compound Anticancer Powder. Methods The experiment was designed by orthogonal test and the content was determined by high performance liquid chromatography (HPLC). Results The optimal extraction process of compound anti-cancer powder was 12 times of solvent, extraction time 1.5h and ethanol concentration 80%. Conclusion The optimum extraction conditions of compound anti-cancer powder dry and ursolic acid are high, and suitable for industrial production.