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AIM: To examine the influence of lipoprotein lipase (LPL) gene polymorphism in ulcerative colitis (UC) patients. METHODS: Peripheral blood was obtained from 131 pa- tients with UC and 106 healthy controls for DNA extrac- tion. We determined LPL gene polymorphisms affecting the enzyme at Ser447stop, as well as HindⅢ and Pvu Ⅱ polymorphisms using PCR techniques. PCR products were characterized by PCR-RFLP and direct sequencing. Polymorphisms were examined for association with clini- cal features in UC patients. Genotype frequencies for LPL polymorphisms were also compared between UC patients and controls. RESULTS: In patients with onset at age 20 years or younger, C/G and G/G genotypes for Ser447stop poly- morphism were more prevalent than C/C genotype (OR = 3.13, 95% CI = 0.95-10.33). Patients with H+/- or H-/-genotype for HindⅢ polymorphism also were more nu- merous than those with H+/+ genotype (OR = 2.51, 95% CI = 0.85-7.45). In the group with H+/+ genotype for HindⅢ polymorphism, more patients had serum triglyc- eride concentrations over 150 mg/dL than patients with H+/- or H-/- genotype (P < 0.01, OR = 6.46, 95% CI = 1.39-30.12). Hypertriglycemia was also more prevalent in patients with P+/+ genotypes for PvuⅡ polymorphism (P < 0.05, OR = 3.0, 95% CI = 1.06-8.50). Genotype fre- quency for LPL polymorphism did not differ significantly between UC patients and controls. CONCLUSION: Ser447stop and HindⅢ LPL polymor- phisms may influence age of onset of UC, while HindⅢ and PvuⅡ polymorphisms influence serum triglyceride in UC patients.
AIM: To examine the influence of lipoprotein lipase (LPL) gene polymorphism in ulcerative colitis (UC) patients. METHODS: Peripheral blood was obtained from 131 pa- tients with UC and 106 healthy controls for DNA extrac tion. We determined LPL gene polymorphisms PCR products were characterized by PCR-RFLP and direct sequencing. Polymorphisms were examined for association with clini-cal features in UC patients. Genotype frequencies for LPL polymorphisms were also compared between UC patients and controls. RESULTS: In patients with onset at age 20 years or younger, C / G and G / G genotypes for Ser447stop poly- morphism were more prevalent than C / C genotype (OR = 3.13, 95% CI = 0.95-10.33). Patients with H +/- or H - / - genotype for HindⅢ polymorphism also were more nu merous than those with H + / + genotype (OR = 2.51, 95% CI = 0.85-7.45). In the group with H + / + genotype for Hind III polymorp hism, more patients had serum triglyc-eride concentrations over 150 mg / dL than patients with H +/- or H - / - genotypes (P <0.01, OR = 6.46, 95% CI = 1.39-30.12). Hypertriglycemia was also more prevalent in patients with P + / + genotypes for PvuII polymorphism (P <0.05, OR = 3.0, 95% CI = 1.06-8.50). Genotype fre quency for LPL polymorphism did not differ significantly between UC patients and controls. CONCLUSION: Ser447stop and HindIII LPL polymor- phisms may influence age of onset of UC, while Hind III and Pvu polymorphisms influence serum triglyceride in UC patients.