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２５％灭幼脲三号在室内用２００～３００ｐｐｍ处理，对双齿多刺蚁ｐｏｌｙｒｈａｃｈｉｓｄｉｖｅｓＳｍｉｔｈ、叉角厉蝽Ｅｏｃａｎｔｈｅｃｏｎａｆａｒｃｅｎｌｌａｔａ（Ｗａｌｆｆ）和蜘蛛的毒性较轻，但对圆梗举腹蚁ＣｒｅｍａｓｔｏｇａｓｔｅｒｄｏｈｒｈｉＭａｙｒ毒性稍重；在灭幼脲中加入多来宝，则对天敌的毒性增加；增效型粉剂对蜘蛛的毒性较重。
25% diflubenzuron III was treated indoors with 200-300 ppm and was less toxic to polyrhachis dives Smith, Eocantheconafarcenllata (Walff) and spiders but slightly more toxic to Cremastogaster dohrhi Mayr, Chlorbenzidine added more to Po, then increased the toxicity of natural enemies; synergistic powder toxicity to spiders heavier.