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目的 :探讨血小板活化因子 (PAF)对离体大鼠肾损害的作用机制和牛磺酸的保护作用 .方法 :实验大鼠均建成离体肾脏灌流模型 ,用K -H灌流液作对照组 ,余两组在K -H液中分别加PAF ,PAF及牛磺酸灌流 ,检测灌流液中的蛋白含量、组织钙、丙二醛 (MDA) .结果 :PAF组漏出蛋白量、组织钙、MDA明显高于对照组 (P <0 0 1) ;牛磺酸组漏出蛋白量明显低于PAF组 (P <0 0 1) ,组织钙、MDA低于PAF组 (P <0 0 5 ) .结论 :PAF是通过其受体影响细胞膜钙通道开放 ,钙内流增加 ,导致钙超载 ,PAF促进细胞膜脂质过氧化损伤肾脏 ;牛磺酸通过调节细胞内钙稳态作用 ,抑制脂质过氧化保护肾脏
OBJECTIVE: To investigate the mechanism of action of platelet activating factor (PAF) on renal injury in isolated rat and the protective effect of taurine. Methods: All experimental rats were established with an isolated kidney perfusion model, and K-H perfusate was used as a control group. The two groups were treated with PAF, PAF and taurine perfusion in K-H fluid, and the protein content, tissue calcium, and malondialdehyde (MDA) in the perfusate were detected. Results: The amount of leaked protein, tissue calcium, and MDA were significantly increased in the PAF group. Compared with the control group (P <0 01), the amount of leaked protein in the taurine group was significantly lower than that in the PAF group (P <0 01), and the tissue calcium and MDA were lower than in the PAF group (P <0 05). PAF is through its receptors affecting the opening of cell membrane calcium channels, increasing calcium influx, leading to calcium overload, PAF promotes lipid peroxidation of the cell membrane to damage the kidney; taurine inhibits lipid peroxidation and protects the kidney by regulating intracellular calcium homeostasis