论文部分内容阅读
目的 通过建立幽门螺杆菌 (H pylori)感染蒙古沙鼠的动物模型 ,观察H pylori及H pylori与N 甲基 N’ 硝基 N 亚甲基胍 (MNNG)联用作用后胃黏膜的组织学改变 ,并观察了维生素C的预防作用。方法 16 0只SPF级蒙古沙鼠随机分为 5组 ,每组 32只。A组单用H pylori菌液灌胃 ;B组在接种H pylori后 4周 ,摄入MNNG水 (2 0 μg/ml) ,连续 30周 ;C组单用MNNG水 (2 0 μg/ml) ,连续 30周 ;D组在B组基础上同时摄入加维生素C的食料 ;E组为空白对照组。各组分别在实验后 12、36、4 8、5 2周各处死 8只 ,取胃黏膜行组织学检查 ,用Warthin Starry银染、PCR和快速尿素酶法检测H pylori。 结果 累计至 5 2周肠化生和异型增生的发生率A组(87 5 % ,6 2 5 % )、B组 (78 1% ,5 6 3% )显著高于C组 (6 2 % ,6 3% )、D组 (15 6 % ,9 4 % )和E组 (0 % ,0 % ) ,P <0 0 1。B组的H pylori感染率从接种后 12周的 10 0 %下降到 5 2周的 6 6 7%。结论 H pylori感染与胃癌发生有关 ,维生素C在预防肠化生和异型增生的癌前病变上有一定作用
Objective To investigate the histological changes of gastric mucosa in H pylori-infected Mongolian gerbils by establishing H pylori-infected H pylori and H pylori with MNNG , And observed the preventive effect of vitamin C. Methods One hundred and sixty SPF Mongolian gerbils were randomly divided into 5 groups (32 in each group). Group A was inoculated intraperitoneally with H pylori bacterium; Group B received MNNG water (20 μg / ml) for 4 weeks after inoculation with H pylori for 30 weeks; Group C was treated with MNNG water (20 μg / ml) For 30 consecutive weeks. Group D was fed with vitamin C on the basis of group B. Group E was a blank control group. Eight rabbits were killed at 12, 36, 48 and 52 weeks after operation in each group. Histological examination was performed on gastric mucosa. Hpylori was detected by silver staining with Warthin Starry, PCR and rapid urease assay. Results The cumulative incidence of intestinal metaplasia and dysplasia in group A was 87.5% (62.5%) in group A and was significantly higher than that in group C (78.1%, 56.3%) in group B 6 3%), D group (15 6%, 94%) and E group (0%, 0%), P 0 01. The infection rate of H pylori in group B decreased from 10 0% at 12 weeks after inoculation to 6 6 7% in 5 2 weeks. Conclusions H pylori infection is related to the occurrence of gastric cancer. Vitamin C plays a role in preventing precancerous lesions of intestinal metaplasia and dysplasia