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白细胞吞噬作用包括趋化、摄食和胞内杀菌等活动,其中以对周围颗粒物质的摄食活动最为基本。对于机体的这一非特异性免疫功能常用纯白细胞进行研究,这不仅需要较多血液,而且在纯化过程中可能使细胞的表面特性因各种物理化学处理而受到损害。使用全血则不存在这些问题,还可以保留许多对吞噬活动十分重要的血清因子。但是迄今报道的全血方法仍多使用常量血液(从0.1ml至数ml不等),在应用中受到一定限制。另外在评价白细胞吞噬能力时多采用吞噬指数和吞噬细胞百分数,由于没有对加入吞噬系统的菌粒密度和反应进行的时间、条件等作出统一规定,不同实验室的测定结果无法相互比较。而且,仅依据吞噬指数值并不能真实反映吞噬细胞的功能活动性,细胞活动性最好用细
Phagocytosis of leukocytes, including chemotaxis, feeding and intracellular bactericidal and other activities, of which the surrounding particulate matter feeding activity is the most basic. The use of pure white blood cells for this nonspecific immune function of the body often requires more blood and may impair the surface properties of the cells due to various physicochemical treatments during the purification process. The use of whole blood does not have these problems, but it also retains many of the serum factors that are important for phagocytosis. However, the whole blood methods reported so far still use constant blood (ranging from 0.1 ml to several ml), and are limited in application. In addition, phagocytic index and percentage of phagocytes are mostly used in evaluating phagocytic capacity of leukocytes. Since there is no unified regulation on the density of phagocytosis and the time and condition of reaction, the results of different laboratories can not be compared with each other. Moreover, phagocytic index alone does not reflect the true functional activity of phagocytes, cell activity is best to use fine