论文部分内容阅读
目的研究甘草查尔酮类活性成分异甘草素(Isoliquiritigenin,ISL)对人宫颈癌Siha细胞增殖和凋亡的影响。方法将细胞接种于96孔板进行培养(每个孔的密度为1×10~4个/mL),待细胞完全贴壁后(24h),将细胞分为ISL 25、50、100、200、300、400μg/mL组及对照组(正常培养的Siha细胞)、顺铂组(顺铂25μg/mL)、DMSO组(20μL DMSO加入5mL的培养基中),用MTT法测定ISL对人宫颈癌Siha细胞增殖的抑制作用。通过流式细胞仪测定细胞凋亡及细胞线粒体跨膜电位的变化。通过Caspase-3、Caspase-8、Caspase-9活性检测试剂盒测定ISL对半胱天冬酶活性的影响。结果 ISL抑制Siha细胞增殖呈时间依赖性及浓度依赖性,可浓度依赖性的升高细胞凋亡率,降低线粒体膜电位,能增强Caspase-3的表达活性,但对Caspase-8、Caspase-9的活性不产生影响。结论 ISL能抑制宫颈癌细胞的增殖,其可能机制是诱导细胞线粒体膜电位途径实现的。
Objective To investigate the effects of isoliquiritigenin (ISL), an active ingredient of licochalcone, on the proliferation and apoptosis of human cervical carcinoma Siha cells. Methods The cells were seeded in 96-well plates (density 1 × 10 ~ 4 / mL). After the cells were fully adhered (24h), the cells were divided into ISL 25, (Control group) (Siha cells in normal culture), cisplatin group (25μg / mL cisplatin), DMSO group (20μL DMSO in 5mL culture medium) Siha cell proliferation inhibition. Flow cytometry was used to determine the changes of apoptosis and mitochondrial transmembrane potential. Caspase-3, Caspase-8, Caspase-9 Activity Assay Kit was used to determine the effect of ISL on caspase activity. Results ISL could inhibit the proliferation of Siha cells in a time-dependent manner and in a concentration-dependent manner. It could increase apoptosis rate and mitochondrial membrane potential in a concentration-dependent manner, and enhance the activity of Caspase-3, but not Caspase-8 The activity does not affect. Conclusion ISL can inhibit the proliferation of cervical cancer cells, and its possible mechanism is to induce mitochondrial membrane potential pathway.