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目的比较不同靶向纳米材料作为基因载体的细胞毒性大小及其转染效率。方法以聚乙烯亚胺(PEI)为对照,化学合成髓鞘碱性蛋白68-86(MBP68-86)-多聚赖氨酸(PLL)-聚乙烯亚胺-聚乙二醇(PEG)接枝支化特异性配体乳铁蛋白(Lf)及PLL-PEI-PEG,并采用MTT法检测细胞毒性,倒置显微镜荧光观察法和流式细胞仪法检测转染效果。结果 MBP68-86-PLL-PEI-PEG-Lf细胞毒性比PLLPEI-PEG及PEI低,三组两两比较差异有统计学意义(P<0.05);MBP68-86-PLL-PEI-PEG-Lf/p AAVEGFP的转染效率为(41.8±0.9)%;PLL-PEI-PEG/pAAV-EGFP的转染效率为(28.4±1.1)%;PEI/p AAVEGFP为的转染效率(20.5±1.2)%,两两比较差异有统计学意义(P<0.05)。结论 MBP68-86-PLL-PEIPEG-Lf和PLL-PEI-PEG可作为基因转染的传输载体,其中MBP68-86-PLL-PEI-PEG-Lf最好。
Objective To compare the cytotoxicity and transfection efficiency of different targeted nanomaterials as gene carriers. Methods Polyethyleneimine (PEI) was used as a control to chemically synthesize myelin basic protein 68-86 (MBP68-86) -polylysine (PLL) -polyethyleneimine-polyethylene glycol (PEG) (Lf) and PLL-PEI-PEG. The cytotoxicity was detected by MTT assay. The transfection efficiency was detected by inverted microscopy and flow cytometry. Results The cytotoxicity of MBP68-86-PLL-PEI-PEG-Lf was lower than that of PLLPEI-PEG and PEI. There was significant difference between the two groups (P <0.05) The transfection efficiency of AAVEGFP was (41.8 ± 0.9)%, that of PLL-PEI-PEG / pAAV-EGFP was (28.4 ± 1.1)%, and that of PEI / p AAVEGFP was (20.5 ± 1.2) (P <0.05). There was a significant difference between every two groups (P <0.05). Conclusion MBP68-86-PLL-PEIPEG-Lf and PLL-PEI-PEG can be used as gene transfer vectors, MBP68-86-PLL-PEI-PEG-Lf is the best.