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细菌和病毒的mRNA一般都是多顺反子,通过对多顺反子的mRNA进行选择性剪切或选择性翻译,从而选择性去除或增加功能性结构域,最终调控蛋白的多样性和基因的功能。但是在哺乳动物细胞中是否也广泛存在多顺反子的mRNA,其是否能从单个mRNA表达多个蛋白并行使多种功能,是正在探索的问题。MAVS(mitochondrial antiviral signaling protein,MAVS)是RIG-I和MDA5下游调控Ⅰ型干扰素产生的关键信号蛋白。首先作者在人的多种细胞系中发现MAVS存在相对分子质量分别为72 kD(FL MAVS)和50 kD(miniMAVS)的两种蛋白,分析发现miniMAVS并不是MAVS的转录异构体翻译的产物,也不是FL MAVS发生蛋白酶解的产物。核糖体谱实验发现,MAVS mRNA
Bacteria and viruses are generally polycistronic mRNAs that selectively remove or increase functional domains through selective cleavage or selective translation of polycistronic mRNAs to ultimately control protein diversity and genes Function However, whether polycistronic mRNAs are also widely present in mammalian cells is an issue that is being explored as to whether they can express multiple proteins from a single mRNA and exert multiple functions. MAVS (MAVS) is the key signaling protein of type I interferon downstream of RIG-I and MDA5. First of all, we found two major proteins of MAVS in MAVS with relative molecular masses of 72 kD (FL MAVS) and 50 kD (miniMAVS). We found that miniMAVS is not the product of translation of MAVS. Nor is it the product of proteolysis of FL MAVS. Ribosomal profiling experiments revealed that MAVS mRNA