论文部分内容阅读
目的探察证实内源性缩醛磷脂对大鼠缺血性脑损伤的保护作用。方法采用行为学探测、经典组化和免疫组化技术对模型大鼠的运动和学习记忆以及纹状体组织病理变化进行形态学探察,实验数据采用SPSS软件统计学处理。结果 1运动行为学探测显示缺血大鼠通过平衡木所需时间明显延长(P<0.05),缩醛磷脂干预的大鼠比缺血大鼠所需时间缩短(P<0.05);握力测试中,缺血大鼠抓握时间缩短(P<0.05),治疗组大鼠抓握时间比缺血组延长(P<0.05)。Morris水迷宫实验中,缩醛磷脂干预的大鼠逃避潜伏期与缺血组相比明显缩短(P<0.05),空间探索经过平台次数明显多于缺血组(P<0.05)。2组织学与免疫组织化学探察显示,缺血大鼠纹状体的背外侧区出现明显的损伤区域,损伤区域中神经元几乎完全丢失,而缩醛磷脂干预组的大鼠则有少数体积较小的神经元幸存。结论内源性缩醛磷脂对大鼠脑缺血损伤有保护作用。
Objective To investigate the protective effect of endogenous plasmalogen on ischemic brain injury in rats. Methods Morphology was used to detect the motor, learning and memory, and histopathological changes of striatum in rats. The experimental data were processed by SPSS software. Results 1 The kinematics test showed that the time required for the rats to pass the balance beam was significantly prolonged (P <0.05), and the plasmalogen-treated rats were shorter than the ischemic rats (P <0.05). In the grip test, The grasping time of ischemic rats was shortened (P <0.05), and the grasping time of rats in treatment group was longer than that of ischemic group (P <0.05). In the Morris water maze test, the escape latency of the plasmalogen-induced rats was significantly shorter than that of the ischemic group (P <0.05), and the number of space exploration platforms was significantly more than that of the ischemic group (P <0.05). 2 histological and immunohistochemical detection showed that ischemic rat striatum dorsal lateral zone obvious injury area, the lesion area of the neurons is almost completely lost, and plasmalogenol intervention group rats have a small number of more than the volume Small neurons survive. Conclusion Endogenous plasmalogen has a protective effect on cerebral ischemic injury in rats.