牛肉绦虫的染色体研究,仅见于国外,Jones认为该虫染色体为2n=20,但未作染色体核型分析,国内至今未见报道。本文采用RPMI—1640培养液加秋水仙索体外培养—低渗处理—空气干燥技术,对牛肉绦虫染色体组型进行了分析。材料与方法用槟榔南瓜子法从病人驱出6条牛肉绦虫活体,经生理盐水反复洗净后,选取成熟节片置于含秋水仙素总浓度为每ml10μgRPMI—1640培养液中,在37℃培养箱连续培养4～5h。在解剖镜下分离 Chromosomes of beef tapeworm, only found in foreign countries, Jones that the pest chromosome 2n = 20, but not for karyotype analysis, so far no reports. In this paper, RPMI-1640 culture medium plus colchicine in vitro culture - hypotonic treatment - air drying technology, the genital beef genitalia was analyzed. Materials and Methods Six beetles were expelled from the patient with the betel nut pumpkin sub-method. After repeated washing with saline, the mature lectin was selected and placed in a medium containing colchicine at a concentration of 10 μg RPMI-1640 and incubated at 37 ° C. Incubator continuous culture 4 ~ 5h. Separation in the dissecting microscope