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本文以抗人精子单克隆抗体作为探针进行人精子特异抗原的寻找工作。用转移免疫印迹法(Western blotting),已由单抗21-2-4识别出一分子量为80K道尔顿的精子蛋白。用人精子去氧胆酸钠提取物走的SDS-聚丙烯酰胺电泳显示80kd是提取物中的主要成分。电泳后将含有8Okd的胶条切下来,再用电泳洗脱法(Electro-elution)可将其回收纯化。为了评价80kd在生殖过程中
In this paper, anti-human sperm monoclonal antibody as a probe for human sperm-specific antigen search. Western blotting has identified a sperm protein with a molecular weight of 80K Daltons from mAb 21-2-4. SDS-polyacrylamide electrophoresis with human sperm deoxycholate extract showed that 80 kd was the major component of the extract. After electrophoresis, the strips containing 8Okd were excised and purified by Electro-elution. In order to evaluate the 80kd in the reproductive process