论文部分内容阅读
应用免疫细胞化学PAP法系统研究了大鼠胚胎13天至成年食道及胃神经激肽A发生发育过程.结果如下:(1)在食道,直至出生前,即胚胎21天才于环肌层及粘膜肌层出现阳性膨体纤维,出生后,随幼鼠的生长发育,相继在上皮内、纵肌层、肌间丛、粘膜下丛出现神经激肽A免疫反应阳性物,其纤维的密度、粗细和着色也逐渐增加,30天时已具备成年的分布特征,其发育主要在生后4周;(2)在胃,于胚胎14天,首先在肌间丛处呈现阳性反应,且发育迅速,阳性反应强,相继在环肌、纵肌、粘膜下层血管壁出现阳性纤维,出生后又于粘膜肌、固有膜及上皮内出现阳性纤维,30天时已和成年鼠的相似,其发育主要在生前1周至生后4周内.提示同一种神经肽在消化道的不同节段其发生不同步,且具不同的发育规律,反映了神经激肽A与消化道的活动有密切的关系.
The immunocytochemical PAP method was used to systematically study the developmental process of rat embryonic day 13 to adult esophageal and gastric neurokinin A. The results are as follows: (1) In the esophagus, until 21 days after embryo was delivered to the muscularis mucosa Muscle layer positive bulge fibers, after birth, with the growth and development of young rats, one after another in the epithelial, longitudinal muscle, myenteric plexus, submucosal plexus appear neurokinin A immunoreactive positive, the fiber density, thickness And coloration also gradually increased, 30 days already have the distribution characteristics of adults, and its development mainly in 4 weeks after birth; (2) in the stomach, on the embryo 14 days, first in the muscular plexus showed a positive reaction, and rapid development, positive Positive reaction, one after another in the ring muscle, longitudinal muscle, submucosal blood vessels appear positive fibers, and after birth in the mucosal muscle, lamina propria and epithelial positive fibers, 30 days and adult mice are similar, and its development mainly in life 1 Week to 4 weeks after birth, suggesting that the same neuropeptide in different segments of the digestive tract asynchrony, and have different developmental rules, reflecting the neurokinin A and digestive tract activity are closely related.