在临床实践中常采用EAC玫瑰花试验来检查,鉴定B淋巴细胞。从末梢血中分离淋巴细胞通常是用密度梯度离心的方法,所分离的淋巴细胞悬液中常常含有不同数量的单核细胞,而单核细胞像B淋巴细胞一样,具有补体受体,因此就防碍了B淋巴细胞的检查。据报导有人采用羰基铁处理细胞悬液,以除掉其中的单核细胞,但除掉的不够 In clinical practice is often used to check the EAC rose test, identification of B lymphocytes. The separation of lymphocytes from peripheral blood is usually performed by density gradient centrifugation. The isolated lymphocyte suspension often contains different numbers of monocytes, which, like B lymphocytes, have complement receptors Hinder B lymphocyte examination. It has been reported that iron carbonyl treatment of cell suspension to remove mononuclear cells therein, but not enough removed