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本文报道了兔大脑缺血再灌流新模型。方法为结扎双侧椎动脉(VA),同时可逆性阻断双侧颈内动脉(ICA)和双侧颈外动脉(ECA),即6-动脉阻断(6AO),60分钟后复流双侧ICA和ECA 120分钟。实验结果显示,脑电活动明显受抑制,脑组织含水量增加至80.41±0.82%,对照组78.13±0.53%((?)±SD),P<0.01。超微结构提示在缺血60分钟时有细胞毒性脑水肿存在,当再灌流120分钟时,细胞毒性和血管源性脑水肿同时存在。与传统的双侧VA和和颈总动脉(CCA)阻断(4AO)模型比较,6AO造成的再灌流损伤较4AO方法更为严重,且不必同时降低全身血压,避免了低血压造成其他重要脏器损伤的可能性。
This article reports a new model of cerebral ischemia and reperfusion in rabbits. Methods: Ligation of bilateral vertebral artery (VA), reversible occlusion of both internal carotid artery (ICA) and bilateral external carotid artery (ECA), 6-artery occlusion (6AO), 60 minutes after the double flow double Side ICA and ECA for 120 minutes. The experimental results showed that EEG activity was significantly inhibited, the water content of brain tissue increased to 80.41 ± 0.82%, and the control group 78.13 ± 0.53% (± SD), P <0.01. The ultrastructure suggests cytotoxic brain edema exists at 60 minutes of ischemia, while cytotoxicity and vasogenic brain edema co-exist at 120 minutes after reperfusion. Compared with the traditional bilateral VA and carotid artery (CCA) block (4AO) model, the reperfusion injury caused by 6AO is more serious than that of the 4AO method, and it is not necessary to reduce systemic blood pressure at the same time to avoid other important dirty The possibility of injury.