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我们于1977年6月开始试用对流免疫电泳法研究出血热病人与黑线姬鼠间的联系,来确定传染源的工作,实验用19例确诊的流行性出血热病人的不同病期和病愈后的血清与疫区黑线姬鼠125只作对流免疫电泳试验,发现6例出血热病人的血清或血浆与黑线姬鼠血清出现沉淀线,其中以病人尹××的血浆出现阳性反应次数最多,同时因获得血量也较多,随即采用此血浆作了进一步系统的试验,今报导如下。材料和方法 (一)病人血浆采自出血热疫区临床确诊为流行性出血热危重型的尹××病员。用此病人血浆作为出血热病原体的抗体。保存于-30℃低温冰箱和4℃冰箱中备用。
In June 1977, we began to test convective immunity electrophoresis to study the association between hemorrhagic fever patients and Apodemus agrarius to determine the source of infection. The experiment was conducted with 19 patients with confirmed epidemic hemorrhagic fever at different stages and recoveries After the serum and the infected area Apodemus 125 only for counter-current immune electrophoresis test and found that 6 cases of hemorrhagic fever serum or plasma and Apodemus serum sediment line, in which the patient Yin × × plasma positive reaction times Up, at the same time because of the amount of blood obtained more, then use this plasma for further systematic testing, this report is as follows. Materials and methods (a) Patient plasma collected from Yin hemorrhagic fever epidemic clinically diagnosed as epidemic hemorrhagic fever type Yin × × patients. Use this patient’s plasma as an antibody to the hemorrhagic fever’s pathogen. Keep at -30 ℃ low temperature refrigerator and 4 ℃ refrigerator spare.