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目的 评估 Ｄ Ｎ Ａ 探针鉴定伴放线放线杆菌（ Ａａ） 的价值，探讨 Ａａ 与牙周炎临床表现间的相关性。方法 ３２ 例成人牙周炎患者５９ 个部位龈下菌斑经 Ａａ 选择性培养基培养后，菌落分别用 Ｄ Ｎ Ａ 探针法及生化法鉴定，并根据探针法的检出率探讨 Ａａ 感染与牙周炎临床参数间的关系。结果 Ｄ Ｎ Ａ 探针法与培养法有一致性（ Ｐ＜０ ．０１ ，ｒ ＝ ０ ．７８ ，但探针法的检出率（２６／５９） 明显高于培养法（１９／５９）（ Ｐ＜ ０ ．０５） ； Ａａ 的检出率与患者的年龄、性别无关，而与牙周组织病损程度相关。结论 Ｄ Ｎ Ａ 探针法优于细胞生化鉴定法， Ａａ 的检出率与成人牙周炎临床参数相关
Objective To evaluate the value of D N A probe in the identification of Actinobacillus actinomycetemcomitans (Aa) and to explore the relationship between Aa and the clinical manifestations of periodontitis. Methods The subgingival plaque in 59 cases of adult periodontitis patients were cultured in Aa selective medium. The colonies were identified by D N A probe method and biochemical method respectively. The detection rate of Aa infection And periodontitis clinical parameters between the relationship. Results The D NA probe method was consistent with the culture method (P <0.01, r = 0.78, but the detection rate of the probe method was significantly higher than that of the culture method (19/59) P <0 .05). The detection rate of Aa was not related to the patient's age and sex, but was related to the degree of periodontal tissue lesions.Conclusion D N A probe method is superior to cell biochemical identification method, the detection rate of Aa and The clinical parameters of adult periodontitis are related