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研究实验性自身免疫性脑脊髓炎 (EAE)及甲基维生素B12治疗中溶血磷脂酸 (LPA)水平的变化。通过注射豚鼠脊髓匀浆和完全弗氏佐剂诱导大鼠发生EAE。用有机溶剂提取、并进一步分离 ,最终用定磷方法测定甲基维生素B12治疗的EAE大鼠血浆及脑溶血磷脂水平 ,未经治疗的EAE大鼠为对照组。发现甲基维生素B12治疗组EAE大鼠与未经治疗EAE大鼠比较 ,恢复期脑与血浆LPA水平增高 ,分别为 30 2 3± 11 5 9μmol L血浆、2 45 4 5±144 89nmol g脑和 9 2 7± 3 2 5 μmol L血浆、5 6 33± 5 6 6nmol g脑 (P <0 0 5 ) ;急性期血浆的LPA增高 ,分别为 10 0 5± 1 70 μmol L血浆和 1 87± 0 5 9μmol L血浆 (P <0 0 5 )。溶血磷脂酸可能参与自身免疫性脑脊髓炎髓鞘的恢复过程。
To study the changes of lysophosphatidic acid (LPA) level in experimental autoimmune encephalomyelitis (EAE) and methylvitamin B12 treatment. EAE was induced in rats by injection of guinea pig spinal cord homogenate and complete Freund’s adjuvant. Extracted with organic solvent and further separated. Finally, the plasma and cerebral lysophospholipid levels of methyl vitamin B12-treated EAE rats were determined by fixed phosphorus method. The untreated EAE rats were used as the control group. The results showed that compared with the untreated EAE rats, the levels of LPA in the brain and plasma of the EAE rats treated with methylvitamin B12 were higher than those of the untreated EAE rats (30 2 3 ± 11 5 9μmol L plasma, 2 454 ± 144 89nmol g brain, 9 2 7 ± 3 2 5 μmol L plasma and 5 6 33 ± 56 6 nmol g brain (P 0 05). LPA in the plasma increased in the acute phase, which were 105 ± 170 micromol L plasma and 1 87 ± 0 5 9 μmol L plasma (P <0 05). Lysophosphatidic acid may be involved in the recovery process of autoimmune encephalomyelitis myelin.