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用体外抑制试验、调理试验和细胞毒试验等方法,对抗恶性疟原虫红内期McAb之功能特性进行了研究。17株McAb中有7株对同步化培养的恶性疟原虫的体外增殖具有明显的抑制作用;抑制强度取决于剂量和作用时间。在McAb—IgG浓度为0.6mg/ml时,上述7株McAb的抑制活性均在94％以上。在6株McAb中,有4株能促进巨噬细胞吞噬疟原虫而发挥调理作用。在7株McAb中,有4株能加强腹腔细胞杀伤和杀死疟原虫而表现出细胞毒活性。结果提示,恶性疟原虫McAb似有“多功能”、“双功能”和”单功能”之分,用“多功能性McAb”纯化的疟疾抗原,在制备疟疾亚单位疫苗方面可能更有意义。
In vitro inhibition test, conditioning test and cytotoxicity test were used to study the functional properties of McAb against Plasmodium falciparum during the red stage. Seven out of 17 McAbs had a significant inhibitory effect on the in vitro proliferation of synchronized P. falciparum; the intensity of inhibition was dependent on dose and duration of action. The McAb-IgG concentration of 0.6mg / ml, the seven McAb inhibitory activity of 94% or more. Among the 6 McAbs, 4 can promote macrophage phagocytosis of Plasmodium and play a role in conditioning. Of the seven McAbs, four showed cytotoxic activity by enhancing intraperitoneal cell killing and killing of Plasmodium. The results suggest that Plasmodium falciparum McAbs may be classified as “multifunctional”, “bifunctional” and “monofunctional.” Malaria antigens purified with “multifunctional McAb” may be more meaningful in the preparation of malaria subunit vaccines.