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用茜素络合物(ALC)对唐鱼(Tanichthys albonubes)进行荧光标记,通过测定唐鱼耳石的标记率、死亡率、SGR和DWG等指标,讨论不同浓度ALC对唐鱼耳石标记效果和对其生长和存活的影响,探讨应用该标记技术追踪唐鱼野外种群迁移行为的可行性。结果表明:在温度28~30℃,浓度50 mg·L-1、80 mg·L-1茜素络合物溶液中浸泡24 h,唐鱼仔鱼、稚鱼死亡率为0,其中80 mg·L-1浓度处理组耳石荧光标记环检出率为100%,而在100mg·L-1浓度下,仔、稚鱼死亡率均达到80%;幼鱼在浓度为80、100 mg·L-1条件下无死亡,其中100 mg·L-1浓度处理组耳石标记环检出率为100%,而150 mg·L-1浓度下幼鱼死亡率为44%;成鱼在100、150 mg·L-1浓度下无死亡,且耳石标记率均为100%,而在200mg·L-1浓度下成鱼死亡率达到100%;此外,稚鱼在80 mg·L-1最适浓度下浸泡24 h后继续饲养90 d,标记组和对照组死亡率均为0,两组体质量、SGR和DWG差异不显著;唐鱼仔稚鱼、幼鱼和成鱼耳石标记的最适茜素络合物浓度分别为80、100和150 mg·L-1。
The albacares of Tanichthys albonubes were fluorescently labeled with alizarin complex (ALC). The effects of different concentrations of ALC on the labeling of otoliths were investigated by measuring the labeling rate, mortality, SGR and DWG of otoliths And its impact on its growth and survival, and explore the application of the marker technology to track the feasibility of migration of wild fish in the pond. The results showed that the mortality of juvenile lettuces and larvae for tilapia was 80% at 80 ℃ · L-1 and 80 mg · L-1 alizarin complex solution for 24 h at 28-30 ℃, In the L-1 group, the detection rate of otolith fluorescence was 100%, while the mortality of juvenile and juvenile fish reached 100% at the concentration of 100 mg · L-1. At the concentration of 80 and 100 mg · L The death rate of larvae was 100% in 100 mg · L-1 treatment group and 44% in juvenile fish at 150 mg · L-1, 150 mg · L-1, no mortality was found and the otolith labeling rate was 100%, while the mortality of adult fish was 100% at the concentration of 200 mg · L-1. In addition, After being soaked for 24 h at the proper concentration, the mortality was kept at 0 for 90 d in both groups. There was no significant difference in the body weight, SGR and DWG between the two groups. The larvae, larvae and otoliths The optimal concentrations of alizarin complex were 80, 100 and 150 mg · L-1, respectively.