采用放射配基竞争抑制实验和收缩功能实验相结合的方法,比较几种α_1肾上腺索受体激动剂对α_1受体两种亚型亲和性的异同。在放射配基竞争抑制实验中,以CEC预温育的海马粗制细胞膜标本中的α_1受体代表α_(1A)亚型,以纯化的肝细胞膜标本中的α_1受体代表α_(1B)亚型,显示去甲肾上腺素(NE)、苯肾上腺索(PE)和A57219对两种α_1受体亚型的pK_1值无明显差异,商甲 Radioligand competition inhibition experiments and contractile function experiments were used to compare the similarities and differences in the affinity between several α 1 adrenoceptor agonists on α 1 receptor subtypes. In radioligand competition inhibition experiments, α_1 (1A) subtype in the crude cell membrane of hippocampus preincubated with CEC represents the α 1A subtype and the α_1 receptor in the purified liver cell membrane sample represents α 1B subunit There was no significant difference in the pK_1 values ​​of the two α_1 receptor subtypes between norepinephrine (NE), adrenal cord (PE) and A57219,