论文部分内容阅读
用离体器官或组织进行实验,必须防止干燥并保持其正常机能。英国生理学家任格(Syd-ney Ringer,1835～1910),于十九世纪八十年代初期开始探索血液中各成份对蛙心收缩的影响,借以为配制可替代血浆或全血的生理溶液提供可能性。他用生理盐液灌流蛙心,得到某种程度的初步成功。但在1883年发现,他的生理盐液不是用蒸馏水,而是用自来水配制的,其中含有少量的钙和其它无机物。发现此错误之后,他立即用蒸馏水配制盐液,重复实验,得到的结果却与1882年所发表的报告有所不同。如果灌流液中无钙,心室收缩不能
Experiments with isolated organs or tissues must prevent drying and maintain their normal function. Syd-ney Ringer (1835-1910), a British physiologist, began to explore the effects of various components of blood on the contraction of frog hearts in the early 1880s to provide a physiological solution that could be used as an alternative to plasma or whole blood possibility. He infused frog hearts with saline and got some initial success. But in 1883 he found that his physiological fluid was not made from distilled water, but from tap water, which contained small amounts of calcium and other minerals. Immediately after discovering the mistake, he prepared the salt solution with distilled water and repeated the experiment, but the result was different from the one published in 1882. If the perfusion fluid without calcium, ventricular contraction can not