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The response of the antioxidant defense system of an intertidal macroalgae Corallina officinalis L. to different dosages of UV-B irradiation was investigated. Results showed that superoxide dimutase (SOD) and peroxidase (POX) increased and then maintained at a relatively stable level when subjected to UV-B irradiation. Catalase (CAT) activity under medium dosage of UV-B irradiation (Muv) and high dosage of UV-B irradiation (Huv) treatments were significantly decreased. Ascorbate peroxidase (APX) activity first remained unaltered and then increased in Huv treatment. In addition, the assay on isozymes was carried out using non-denaturing polyacrylamide gel electrophoresis (PAGE). The activities of some SOD isoforms were altered by UV-B. Two new bands (POX V and POX Ⅶ) appeared upon exposure to all three UV-B dosages. CAT Ⅲ activity was increased by low dosage of UV-B irradiation (Luv),whereas CAT Ⅲ and CAT Ⅳ disappeared when the alga was exposed to Muv and Huv. Two bands of APX (APX Ⅵ and APX Ⅶ)were increased and a new band (APX X) was observed under Huv exposure. H2O2 and thiobarbituric acid reacting substance (TBARS)increased under Muv and Huv treatments. Overall, UV-B protection mechanisms are partly inducible and to a certain extent sufficient to prevent the accumulation of damage in C officinalis.