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从河南省不同地区采集腹泻死亡仔猪进行病原分离鉴定,通过对细菌形态观察、PCR检测、生化试验以及小鼠毒力试验鉴定出了8株具有致病性大肠杆菌。通过WHO推荐的Kirby-Bauer(K-B)法测定8株大肠杆菌对21种抗菌药物的耐药性,并进一步对分离的8株大肠杆菌进行运动性以及生物被膜形成特性进行比较。生化特性结果表明8株大肠杆菌对各种糖的分解能力无明显差异;耐药性结果表明8株大肠杆菌均是多重耐药菌株,并且8株大肠杆菌在半固体培养基上均具有运动性,形成较明显的运动圈;生物被膜表型检测结果表明:有4株大肠杆菌具有较强的生物膜形成能力。本试验为进一步研究河南地区大肠杆菌的流行病学和探讨生物被膜和致病性、毒力之间的相关性奠定基础。
Eight piglets with dead diarrhea were collected for pathogen isolation and identification in different areas of Henan Province. Eight pathogenic Escherichia coli strains were identified through morphological observation, PCR, biochemical tests and virulence tests in mice. The antibiotic resistance of 8 strains of Escherichia coli against 21 antibiotics was determined by Kirby-Bauer (K-B) method recommended by the WHO, and further comparison was made between the kinetics of 8 strains of Escherichia coli isolated and the biofilm formation characteristics. The results of biochemical characterization showed that there was no significant difference in the ability of various strains of Escherichia coli to disintegrate various kinds of sugars. The drug resistance results showed that all of the eight strains of Escherichia coli were multi-drug resistant strains, and all of the eight strains of Escherichia coli were motile in semi-solid medium , Forming a more obvious movement circle; biofilm phenotype test results show that: four strains of Escherichia coli has a strong biofilm formation ability. This study lays the foundation for further study on the epidemiology of Escherichia coli and the correlation between biofilm and pathogenicity and virulence in Henan province.