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目的建立HPLC波长切换法测定藿香清胃胶囊中栀子苷、升麻素苷和5-O-甲基维斯阿米醇苷含量。方法色谱柱为Agilent HC-C18(4.6 mm×250 mm,5μm);流动相为甲醇-水,梯度洗脱,流速为1.0m L·min-1,柱温:30℃,波长切换时间序列采样:0~12 min为238 nm;12~45 min为254 nm。结果栀子苷进样量在0.382~3.819μg范围内线性关系良好(r=0.9999),平均回收率为99.66%,RSD为1.5%(n=9);升麻素苷进样量在0.011~0.108μg范围内线性关系良好(r=0.9997),平均回收率为99.53%,RSD为1.6%(n=9);5-O-甲基维斯阿米醇苷进样量在0.010~0.097μg范围内线性关系良好(r=0.9998),平均回收率为100.12%,RSD为1.8%(n=9)。结论该方法准确、快捷、可靠,为全面控制藿香清胃胶囊的质量提供参考。
OBJECTIVE To establish a HPLC wavelength-switching method for the determination of geniposide, ciminoside and 5-O-methylvivinolinoside in Huoxiang Qingwei Capsule. Methods The column was Agilent HC-C18 (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of methanol-water with gradient elution at a flow rate of 1.0 mL · min-1. The column temperature was 30 ℃ and the wavelength- : 238 nm at 0-12 min and 254 nm at 1245 min. Results The calibration curve of geniposide was linear in the range of 0.382 ~ 3.819 μg (r = 0.9999) with an average recovery of 99.66% and a RSD of 1.5% (n = 9) (R = 0.9997), the average recovery was 99.53%, RSD was 1.6% (n = 9); the injection volume of 5-O-methyl-Wvisainin was between 0.010 ~ 0.097μg The linear relationship was good (r = 0.9998) with an average recovery of 100.12% and RSD of 1.8% (n = 9). Conclusion The method is accurate, rapid and reliable, providing a reference for the comprehensive control of the quality of Huoxiang Qingwei Capsule.