论文部分内容阅读
给成年雄性大鼠按每100g体重每日注射盐酸酚苄明(PBZ)70μg,共5周,对照组注射生理盐水。处理组每只雄鼠与动情前期雌鼠合笼,观察其生育力。合笼第二天早晨检查雌鼠阴道内阴栓及精子,并记录合笼时雄鼠与雌鼠交配的性行为作为性功能的指征。合笼后5天(合笼期间继续给药),在乙醚麻醉下杀死雄鼠,收集血清和睾丸组织,测定睾酮水平;取附睾和输精管的精子进行精子计数和评价其活力;称重附睾、睾丸和附性腺。
Adult male rats were injected daily with 70 μg of phenoxybenzamine hydrochloride (PBZ) per 100 g body weight for 5 weeks, and saline was injected into the control group. The treatment group each male and estrus females caged cage to observe the fertility. The next morning, the cage was checked for female vaginal suppository and sperm, and the sexual behavior of males and females was recorded as an indication of sexual function. The rats were sacrificed under ether anesthesia 5 days after the cages were caged, and the serum and testicular tissues were collected to test the level of testosterone. The sperms of the epididymis and vas deferens were counted and their sperm motility was measured. , Testis and gonads.