论文部分内容阅读
研究了纳米硒对睡眠剥夺(SD)小鼠(Mus musculus)认知功能的影响,并探讨其作用机制。将120只雄性昆明小鼠随机分成两批,第一批24只分为3组:对照组(NC)、亚硒酸钠组(SE)和纳米硒组(NS),分别给予硒浓度为4μg/ml的亚硒酸钠和纳米硒溶液每只0.5ml/d,NC组给等体积蒸馏水,连续30d,第31天测定SE和NS两组小鼠的血硒及全血GSH-Px活性,评价两种硒源的生物利用性;第二批96只分为4组:对照组(N-SeC),纳米硒低、中、高剂量组(L、M、H),L、M和H组分别给予硒浓度为2μg/ml、4μg/ml、8μg/ml的纳米硒溶液每只0.5ml/d,N-SeC组给予同体积蒸馏水,连续30d。第二批小鼠每组又各自分为4小组:SD对照组(SDC)及SD18h、SD36h、SD54h组,采用单平台水环境法(SPM)制作小鼠SD模型。在SD后,N-SeC、L、M和H组利用Y-型迷宫试验测定认知能力,同时测定小鼠大脑GSH-Px、NO、MDA含量。结果表明,纳米硒对GSH-Px活性的提高优于传统硒源亚硒酸钠,但血硒无显著差异;与SDC组比较,SD降低了小鼠的认知能力及大脑GSH-Px活性,提高了NO和MDA含量;与N-SeC比较,纳米硒使SD小鼠的认知功能得到改善,大脑GSH-Px活性提高,MDA和NO含量下降。上述结果表明,纳米硒能够改善SD小鼠的认知功能,这可能与其提高大脑GSH-Px活性并降低了自由基对大脑神经的损害有关。
The effect of nano-selenium on cognitive function in sleep deprivation (SD) mice (Mus musculus) was studied and its mechanism was explored. 120 male Kunming mice were randomly divided into two groups, the first 24 were divided into three groups: control group (NC), sodium selenite group (SE) and nano selenium group (NS), were given selenium concentration of 4μg / ml sodium selenite and nano-selenium solution each 0.5ml / d, NC group to the same volume of distilled water for 30 days, the determination of selenium and serum GSH-Px activity of SE and NS mice on the 31st day, The second 96 batches were divided into 4 groups: control group (N-SeC), low, middle and high dose Nano Selenium (L, M, H), L, M and H Group were given selenium concentration of 2μg / ml, 4μg / ml, 8μg / ml of nano-selenium solution each 0.5ml / d, N-SeC group were given the same volume of distilled water, continuous 30d. The second group of mice were divided into 4 groups: SD control group (SDC) and SD18h, SD36h and SD54h groups. SD mouse model was established by single platform water environment method (SPM). After SD, cognitive ability was measured by Y-type maze test in N-SeC, L, M and H groups, and the contents of GSH-Px, NO and MDA in the brain were measured simultaneously. The results showed that the activity of GSH-Px by nano-selenium was better than that of the traditional selenium source, but selenium had no significant difference. Compared with the SDC group, SD decreased the cognitive ability and GSH-Px activity of the brain, Compared with N-SeC, nano-selenium improved the cognitive function of SD mice, increased the activity of GSH-Px in the brain, and decreased the content of MDA and NO. The above results show that nano-selenium can improve cognitive function in SD mice, which may be related to its activity of increasing GSH-Px in the brain and decreasing the damage of free radicals to the brain.