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目的：建立放射诱导胃癌细胞凋亡模型，为进一步研究细胞凋亡分子机理打下基础。方法：用直线加速器12Gy9MeV 照射培养的人胃癌细胞株SGC－7901，诱导凋亡发生，检测DNA条带变化，电镜观察凋亡小体的出现，流式细胞仪检测凋亡率及细胞周期和细胞内Ca2＋、cAMP浓度。结果：照射后2小时，即有凋亡细胞出现，在72小时达高峰，胃癌细胞DNA结构断裂，电泳呈梯状带形。胃癌细胞形态学改变出现膜小泡和凋亡小体形成等凋亡细胞特征。FCM检测出现典型的凋亡峰，以G2～M期凋亡率为最高，照射后2、24、48、72小时凋亡率分别为11．26％、38．39％、50．59％及59．47％，胞内Ca2＋和cAMP浓度变化也随凋亡率增加而升高。结论：照射12Gy9MeV诱导胃癌细胞株SGC－7901发生凋亡，凋亡率在72小时达高峰。胞内Ca2＋和cAMP浓度变化起了相当重要的作用。
OBJECTIVE: To establish a radiation-induced apoptosis model of gastric cancer cells and lay a foundation for the further study of molecular mechanisms of apoptosis. METHODS: Human gastric cancer cell line SGC-7901 was irradiated with linear accelerator 12Gy9MeV, and apoptosis was induced. DNA bands were detected. Electron microscopy was used to observe the appearance of apoptotic bodies. Flow cytometry was used to detect apoptosis rate and cells. Cycle and intracellular Ca2+, cAMP concentrations. Results: Two hours after irradiation, apoptotic cells appeared and peaked at 72 hours. The DNA structure of gastric cancer cells was broken, and the electrophoresis was ladder-shaped. Morphological changes of gastric cancer cells showed apoptotic cell features such as vesicles and apoptotic bodies. The typical apoptotic peaks were detected by FCM, and the apoptotic rate was highest in G2-M phase. The apoptotic rates were 11.26%, 38.39%, and 50.59% at 2, 24, 48, and 72 hours after irradiation. 59.47%, intracellular Ca2+ and cAMP concentrations also increased with increasing apoptotic rate. Conclusion: The apoptosis of gastric cancer cell line SGC-7901 induced by 12Gy9MeVwas apoptotic, and the apoptotic rate peaked at 72 hours. Changes in intracellular Ca2+ and cAMP concentrations play an important role.