论文部分内容阅读
目的:分析广藿香经组织培养技术获得的再生植株中百秋里醇和广藿香酮的含量,并与扦插药材进行比较,为广藿香药材的组织培养条件以及品质鉴定提供实验依据。方法:采用GC法分别对3批组培与3批扦插广藿香药材中百秋里醇和广藿香酮进行定量分析。色谱条件为:安捷伦DB-1毛细管柱(30m×0.25mm,0.25μm);FID检测器;程序升温:初始温度120℃,停留3min后以每分钟10℃的速度升至220℃;载气为高纯氦气,流速:1mL.min-1,进样口温度和检测器温度均为230℃,分流比:50:1。结果:组培药材中百秋里醇和广藿香酮的含量与扦插药材相比,无显著性差异。结论:组织培养适于广藿香药材的繁殖,并能保证药材的质量。
OBJECTIVE: To analyze the contents of periqiuolol and patchoulenone in regenerated plants obtained through tissue culture of Patchouli and compared with those of cutting medicinal materials, and provide the experimental basis for the tissue culture conditions and quality identification of the patchouli medicinal materials. Methods: GC method was used to quantitatively analyze the contents of periqiuolol and patchoulenone in three batches of tissue culture and three batches of patchouli medicinal materials. Chromatographic conditions: Agilent DB-1 capillary column (30m × 0.25mm, 0.25μm); FID detector; program temperature: initial temperature of 120 ℃, stay 3min at 10 ℃ per minute speed up to 220 ℃; High-purity helium, flow rate: 1mL.min-1, inlet temperature and detector temperature were 230 ℃, split ratio: 50: 1. Results: There was no significant difference in the content of periol and patchoulenones in tissue culture materials compared with the cuttings. Conclusion: Tissue culture is suitable for the propagation of patchouli medicinal materials and can ensure the quality of medicinal materials.