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Wilm瘤基因(WT1)在白血病细胞中高表达提示其可作为一种广谱的白血病标志,但早期由于定性或半定量RT PCR技术的局限性使其在微小残留病 (MRD)诊断中的价值难以得到一致认可。为此我们采用实时荧光定量 (FQ) RT PCR技术,检测了不同类型白血病患者WT1基因的表达;并对部分急性
The high expression of Wilm’s oncogene (WT1) in leukemia cells suggests that it may serve as a broad-spectrum markers of leukemia, but its early limitations due to the limitations of qualitative or semi-quantitative RT-PCR make it difficult to diagnose MRD Be unanimously approved To this end, we used real-time fluorescence quantitative (FQ) RT PCR technology to detect WT1 gene expression in different types of leukemia patients;