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目的:探讨高功率脉冲微波(high power pulse microwave,HPPM)辐射对小鼠脑、肝、脾氧化应激效应及巨噬细胞氧耗的影响。方法:雄性昆明小鼠30只,随机分为3组(每组10只):正常对照组(A),辐射1次组(B),辐射3次组(C)。采用S波段HPPM,平均表面功率10 mW/cm2;照射时间2 min/次,隔日辐射1次。末次辐射完毕,立即处死小鼠,取脑、肝、脾、心、肾脏制作匀浆。采用比色法测定脏器匀浆的超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、谷胱甘肽(GSH)含量。收集大鼠腹腔巨噬细胞,调整浓度为1×107/ml,将细胞悬液分为两组(n=12):对照组和HPPM组。微波辐射持续时间15 min。结果:HPPM作用后,与辐射1次组和对照组比,辐射3次组脑组织SOD活性、GSH含量明显降低,有统计学差异,而CAT、GSH-px活性变化不明显。脾组织GSH含量在HPPM作用后均明显降低,其中HPPM辐射1次组降低程度较HPPM辐射3次组更为明显,脾组织SOD活性变化不明显。肝组织SOD活性、GSH含量在HPPM作用后均呈辐射剂量依赖性降低,CAT活性变化不明显。而心、肾组织在辐照前后各指标均无显著性变化。巨噬细胞在HPPM作用15 min后,氧耗明显增加。结论:高功率脉冲微波辐射可以引起小鼠脑、肝、脾等脏器产生氧化应激的改变,体外巨噬细胞氧耗增加。
Objective: To investigate the effect of high power pulse microwave (HPPM) radiation on the oxidative stress in brain, liver and spleen of mice and the oxygen consumption of macrophages. Methods: Thirty male Kunming mice were randomly divided into three groups (n = 10): normal control group (A), radiation group (B) and radiation group (C). Using S-band HPPM, the average surface power of 10 mW / cm2; irradiation time 2 min / times, every other day radiation. The last radiation is completed, immediately sacrificed mice, brain, liver, spleen, heart, kidney production of homogenate. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) were measured by colorimetry. The peritoneal macrophages of rats were collected and adjusted to a concentration of 1 × 10 7 / ml. The cell suspensions were divided into two groups (n = 12): control group and HPPM group. Microwave radiation duration 15 min. Results: Compared with the primary radiation group and the control group, the activity of SOD and the content of GSH in the brain tissue of the irradiated group were significantly lower than those of the primary irradiated group and the control group. The activity of CAT and GSH-px did not change obviously. The content of GSH in spleen tissue decreased significantly after HPPM treatment. The decrease of HPPM radiation in the first group was more obvious than that in the HPPM group, and the change of SOD activity in the spleen was not obvious. The activity of SOD and the content of GSH in liver tissue all decreased in a dose-dependent manner after HPPM, but the activity of CAT did not change obviously. However, no significant changes were observed in heart and kidney before and after irradiation. Macrophages in the role of HPPM 15 min, the oxygen consumption increased significantly. Conclusion: High-power pulsed microwave radiation can cause changes of oxidative stress in the brain, liver and spleen of mice, and increase the oxygen consumption of macrophages in vitro.