论文部分内容阅读
目的建立参七胶囊的质量标准。方法采用薄层色谱法鉴别参七胶囊中的丹参和三七;采用HPLC法测定丹参酮ⅡA和丹酚酸B的含量。结果丹参酮ⅡA色谱柱:安捷伦ZORBAX SB-C18(250 mm×4.6 mm,5μm),流动相:甲醇-水(体积比为82∶18),流速:1.0 m L·min-1,检测波长:270 nm,平均回收率:100.5%,RSD=1.7%(n=6)。丹酚酸B色谱柱:Kromsail C18(150 mm×4.6 mm,5μm),流动相:甲醇-乙腈-甲酸-水(体积比为49∶16∶2∶132),流速:1.0 m L·min-1,检测波长:286 nm,平均回收率:100.4%,RSD=1.3%(n=6)。结论本法可用于参七胶囊的质量控制。
Objective To establish the quality standard of Shenqi capsule. Methods Thin layer chromatography was used to identify Salvia miltiorrhiza and Panax notoginseng in Shenqi capsule. The contents of tanshinone Ⅱ A and salvianolic acid B were determined by HPLC. Results Tanshinone Ⅱ A column was connected with Agilent ZORBAX SB-C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol and water (volume ratio 82:18), the flow rate was 1.0 m L · min -1 and the detection wavelength was 270 nm, average recovery: 100.5%, RSD = 1.7% (n = 6). Salvianolic acid B column: Kromsail C18 (150 mm × 4.6 mm, 5 μm) with a mobile phase of methanol-acetonitrile-formic acid-water at a volumetric ratio of 49:16:2:13 and a flow rate of 1.0 m L · min- 1, detection wavelength: 286 nm, average recovery: 100.4%, RSD = 1.3% (n = 6). Conclusion This method can be used for the quality control of Shenqi capsule.