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目的比较克山病患者与健康者血清蛋白表达谱,探讨克山病发病机制,寻找与克山病发生相关差异蛋白。方法从克山病病区选择8例慢型克山病,病区对照以及非病区对照各8例,应用双向凝胶电泳技术分离克山病患者同健康者的差异蛋白点,基质辅助激光解吸电离-飞行时间质谱鉴定差异蛋白。结果凝胶图像分析显示9个差异蛋白质点,经质谱鉴定确定8种蛋白。3种蛋白在克山病组较非病区健康组上调,其功能主要与脂类代谢,免疫调节,凋亡抑制有关。3种蛋白下调,主要与细胞内铁离子平衡密切相关。克山病组较病区健康组2种蛋白表达上调,主要与蛋白酶抑制等功能有关。结论该研究发现触珠蛋白、血清白蛋白和α1-抗胰蛋白酶、转铁蛋白、α2-HS糖蛋白可作为克山病的诊断与预后的候选生物学指标,具有重要意义。
Objective To compare the serum protein expression profiles of patients with Keshan disease and healthy subjects and to explore the pathogenesis of Keshan disease and to search for the differential proteins related to Keshan disease. Methods 8 cases of Keshan disease, 8 cases of control group and 8 cases of non-disease control group were selected from Keshan disease area. Two-dimensional gel electrophoresis technique was used to separate the differentially expressed protein spots of Keshan disease from healthy subjects, Desorption ionization - time of flight mass spectrometry identification of differential proteins. Results Gel image analysis revealed nine differential protein spots identified by mass spectrometry identified eight proteins. The three proteins were up-regulated in Keshan disease group than those in non-disease group, and their functions were mainly related to lipid metabolism, immune regulation and apoptosis inhibition. 3 kinds of protein down, mainly with intracellular iron ion balance is closely related. Keshan disease group than the ward health group two kinds of protein expression, mainly associated with protease inhibition and other functions. Conclusion The study found that haptoglobin, serum albumin and α1-antitrypsin, transferrin, α2-HS glycoprotein Keshan disease can be used as a candidate biological indicators of prognosis, is of great significance.