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长江水产研究所鱼类遗传育种研究室自1987年承担农业部04课题研究任务以来,进行了淡水养殖鱼类细胞电激融合实验。使用的仪器由Baekon-2000型电融合仪的电源,自制电极和电激融合槽,以及SV-8型立体显微镜组成。1988年计进行了团头鲂囊胚细胞与团头鲂未受精卵,草鱼囊胚细胞与团头鲂受精卵两个组合,11批电激融合实验。共获得了81个细胞融合原肠胚,并从中孵化出50尾鱼苗。初步核型分析结果表明,细胞融合鱼多为嵌合体。
Fisheries Research Institute of Yangtze River Fisheries Genetic Breeding Laboratory since 1987 to undertake the task of the Ministry of Agriculture 04 research task, conducted a freshwater aquaculture fish cells electrostimulation experiments. The instrument used was composed of a Baekon-2000 type electrofusion instrument, a self-made electrode and an electrofusion cell, and a SV-8 stereomicroscope. In 1988, the total number of blastocyst cells and breams, unfertilized eggs, grass carp blastocyst cells and bumblebee zygotes were combined in two batches, 11 batches of electroporation experiments. Totally 81 cells were fused with gastrula and 50 fry were hatched from them. Preliminary karyotyping results show that the cell fusion fish mostly chimera.