论文部分内容阅读
目的探讨肺炎链球菌稳定L型变异的分子基础以及聚合酶链反应在肺炎链球菌稳定L型检测中的应用。方法应用肺炎链球菌核酸外切酶基因 (exoA)特异性的引物 ,以聚合酶链反应检测非高渗透压培养基传代培养的肺炎链球菌稳定L型纯培养物的染色体DNA。结果肺炎链球菌稳定L型的染色体DNA经PCR扩增后电泳可见形成与其亲代细菌型一致的分子量为 5 30bp的DNA带。结论肺炎链球菌稳定L型仍保留了与其亲代细菌型一致的exoA基因 ,因此可用于对肺炎链球菌稳定L型进行菌种的鉴定
Objective To investigate the molecular basis of stable L-type variant of Streptococcus pneumoniae and the application of polymerase chain reaction in stable L-type detection of Streptococcus pneumoniae. Methods Chromosomal DNA of Streptococcus pneumoniae stable L-type pure culture subcultured in non-hypertonic medium was detected by polymerase chain reaction (PCR) using primers specific for exoA. Results Stable L-type chromosomal DNA of Streptococcus pneumoniae was amplified by PCR, and then a DNA band with a molecular weight of 5 30 bp was formed consistent with its bacterium’s bacterial type. Conclusions Streptococcus pneumoniae stable L-form still retains the exoA gene consistent with its parental bacterium, so it can be used to identify Streptococcus pneumoniae stable L-type strains