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本文作者对6种多异戊烯乙酸和6种胞壁酰二肽(MDP)衍生物的免疫学活性进行了研究。取350～400g的雌性豚鼠,足垫免疫细菌α-淀粉酶(BαA)抗原,总剂量为300μg/0.4ml,抗原溶剂是弗氏不完全佐剂(FIA)。免疫时对照组不用佐剂,试验组使用不同种类的佐剂(100μg)。4周后从后眼窝静脉丛取血,用酶学方法测定抗BαA抗体。取血后24小时皮内注射100、50或10μg BαA,于注射后4、24和48小时观察皮肤反应。抗原为单偶氮苯胂酸盐(ABA)-N-乙酰-L-酪氨酸(50μg),免疫两周后皮内注射ABA-牛血清白蛋白(BSA)(50或10μg)并于
The authors studied the immunological activity of six polyvaleric acetic acids and six muramyl dipeptide (MDP) derivatives. Take 350 ~ 400g of female guinea pigs, foot pad immune bacteria α-amylase (BαA) antigen, a total dose of 300μg / 0.4ml, the antigen solvent is Freund’s incomplete adjuvant (FIA). In the control group, no adjuvant was used for immunization, and different types of adjuvant (100 μg) were used in the test group. Blood was taken from the retro-orbital venous plexus 4 weeks later and anti-BαA antibodies were determined enzymatically. Twenty-four hours after the blood was taken, 100, 50 or 10 μg of BαA were injected intradermally, and the skin reaction was observed at 4, 24 and 48 hours after the injection. The antigen was monoazo phenyl-formate (ABA) -N-acetyl-L-tyrosine (50 μg) and injected intradermally with either ABA-BSA (50 or 10 μg)