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目的探讨葡萄籽原花青素(GSPE)对大鼠肾缺血-再灌注损伤的保护作用及其机制。方法选用健康雄性Wistar大鼠36只,随机分为3组:假手术组(Sham组)、肾缺血-再灌注损伤组(RIRI组)、葡萄籽原花青素预处理组(GSPE组),建立大鼠肾缺血-再灌注损伤模型,于肾缺血-再灌注2 h、24 h分别检测血清肌酐(SCr)、血清尿素氮(BUN)含量的变化及血清和组织丙二醛(MDA)的含量及谷胱甘肽光氧化物酶(GSH-Px)活性的变化。结果与Sham组相比,IRIR组和GSPE组血清SCr、BUN含量升高(P<0.01),血清及肾组织MDA含量升高(P<0.01),GSH-Px活性降低(P<0.01);与IRIR组相比,GSPE组的SCr、BUN含量降低(P<0.01),血清及肾组织MDA水平降低(P<0.05),GSH-Px活性升高(P<0.01)。结论在肾缺血-再灌注损伤过程中,葡萄籽原花青素能改善肾功能,其机制可能与抗自由基损伤和减轻脂质过氧化有关。
Objective To investigate the protective effect of grape seed proanthocyanidins (GSPE) on renal ischemia-reperfusion injury in rats and its mechanism. Methods Thirty - six healthy male Wistar rats were randomly divided into three groups: Sham group, RIRI group and GSPE group. The changes of serum creatinine (SCr), serum urea nitrogen (BUN) and serum and tissue malondialdehyde (MDA) levels were detected at 2, 24 h after renal ischemia-reperfusion injury in rat kidney ischemia-reperfusion injury model. Content and glutathione oxidase (GSH-Px) activity changes. Results Compared with Sham group, the levels of serum SCr and BUN were increased in IRIR group and GSPE group (P <0.01), and MDA content in serum and kidney tissue was increased (P <0.01) and GSH-Px activity was decreased (P <0.01). Compared with IRIR group, the levels of SCr and BUN in GSPE group decreased (P <0.01), MDA in serum and kidney tissue decreased (P <0.05) and GSH-Px activity increased (P <0.01). Conclusion During the process of renal ischemia-reperfusion injury, grape seed proanthocyanidins can improve renal function, and its mechanism may be related to the anti-free radical damage and the reduction of lipid peroxidation.