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目的研究以交联明胶为壁材的复合维生素微胶囊的制备工艺及体外释放行为。方法在过氧化氢存在下,以阿魏酸为交联剂,通过辣根过氧化物酶催化,制备交联明胶,考察反应条件对交联度的影响;然后以交联明胶为壁材,以盐酸硫胺素(维生素B1)、核黄素(维生素B2)、盐酸吡哆醇(维生素B6)、叶酸、烟酰胺的混合物为芯材,采用喷雾干燥工艺,按不同壁材/芯材比,制备复合维生素微胶囊。用扫描电镜和激光粒度仪,对微胶囊进行了表征。用荧光分光光度计,对微胶囊的包埋率、荷载量以及在模拟胃液和模拟肠液中的释放行为,进行了测试和分析。结果在温度为40℃、pH值为8.0、明胶浓度为9%(W/V)、阿魏酸浓度为40 mmol.L-1、交联时间为24 h的反应条件下,交联明胶的交联度较高,达到10%。以此交联明胶为壁材制备的微胶囊,其中维生素的包埋率在85%以上;少数呈表面光滑的圆球形,大多数表面有凹陷;平均粒径为15.27μm;当壁材/芯材为10/1(W/W)时,维生素在模拟胃液和模拟肠液中完全释放的时间,分别为30和16 min,都比明胶为壁材时相应延长。结论以交联明胶为壁材,采用喷雾干燥工艺,可以制备出包埋率高、具有一定的缓释性能的复合维生素微胶囊。
Objective To study the preparation process and in vitro release behavior of multivitamin microcapsules with crosslinked gelatin as wall material. Methods Ferulic acid was used as crosslinking agent in the presence of hydrogen peroxide to prepare crosslinked gelatin by horseradish peroxidase. The effects of reaction conditions on the degree of crosslinking were investigated. Then, With thiamine hydrochloride (vitamin B1), riboflavin (vitamin B2), pyridoxine hydrochloride (vitamin B6), folic acid, nicotinamide mixture as the core material, using spray drying process, according to different wall material / , Preparation of multivitamin microcapsules. The microcapsules were characterized by scanning electron microscopy and laser particle sizer. Fluorescence spectrophotometer was used to test and analyze the microcapsule embedding rate, the loading capacity and the release behavior in simulated gastric juice and simulated intestinal fluid. Results Under the reaction conditions of the temperature of 40 ℃, the pH value of 8.0, the gelatin concentration of 9% (W / V), the concentration of ferulic acid of 40 mmol.L-1 and the crosslinking time of 24 h, High degree of crosslinking, up to 10%. So as to cross-link the gelatin as the microcapsule prepared from the wall material, wherein the embedding rate of vitamins is over 85%; a few are spherical with a smooth surface, most of the surface is dented; the average particle diameter is 15.27μm; When the material was 10/1 (W / W), the time of complete release of vitamins in simulated gastric fluid and simulated intestinal fluid was 30 and 16 min, respectively, longer than that of gelatin for wall material. Conclusion The cross-linked gelatin as the wall material, using spray-drying process, can be prepared with high embedding rate, with certain sustained-release properties of multivitamin microcapsules.