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为研究硫代葡萄糖苷(Glucosinolate,简称硫苷)对辐射损伤小鼠的保护作用,预先给小鼠口服不同剂量的硫苷粗提物,两周后给予2Gy~(12)C~(6+)离子束全身均匀辐照。辐照后8h内用流式细胞仪检测受辐照小鼠脑、肝、肺细胞在各细胞周期的百分率,用彗星电泳技术检测受辐射小鼠脑、肝、肺细胞的拖尾率和拖尾长度。与单纯辐照未给药组相比,口服较低剂量的硫苷粗提物,可以使~(12)C~(6+)离子束全身辐照后小鼠的脑、肝、肺细胞中G_0/G_1期细胞百分比显著降低(p<0.05),G_2/M期和S期的细胞百分比明显上升(p<0.05),受辐照损伤细胞的拖尾率和拖尾长度也显著降低(p<0.05),而在中、高剂量组间差异都不显著,无统计学意义。研究表明,较低剂量的硫苷能够降低~(12)C~(6+)离子束辐照时对小鼠脑、肝、肺细胞的损伤,说明硫苷对小鼠辐射损伤具有一定的保护作用。
In order to study the protective effect of glucosinolate (Glucosinolate) on radiation-injured mice, mice were given orally different doses of crude glucosinolate, and 2 weeks later, 2Gy~(12)C~(6+) was given. ) The ion beam is uniformly irradiated throughout the body. The percentage of brain, liver, and lung cells in irradiated mice was measured by flow cytometry within 8 hours after irradiation. The tailing rate and drag of brain, liver, and lung cells in irradiated mice were detected by comet electrophoresis. Tail length. The oral administration of a lower dose of the crude glucosinolate resulted in the whole body irradiation of the (12)C 6+ ion beam in the brain, liver, and lung of the mice compared with the unirradiated group irradiated alone. The percentage of G 0 /G 1 phase cells was significantly decreased (p<0.05), the percentage of cells in G 2 /M phase and S phase was significantly increased (p<0.05), and the tailing rate and tail length of cells damaged by irradiation were also significantly decreased (p <0.05), but there was no significant difference between the medium and high dose groups, and there was no statistical significance. Studies have shown that lower doses of glucosinolates can reduce the damage of brain, liver and lung cells in mice irradiated by ~(12)C~(6+) ion beam, indicating that glucosinolates have protective effects on radiation damage in mice. effect.