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本文介绍一种改进的测定磺胺Bratton-Marshall方法。此法不仅试剂容易得到,而且在边远地区设备简陋的实验室也能使用。试剂:1.亚硝酸钠溶液(用蒸馏水溶解),0.03mmol/l或0.2mg/100ml或0.2%(W/V)。2.浓盐酸。3.Bratton-Marshall溶液(BM):将20mg N-(1-萘基)乙二胺双氢化氯溶解在20ml蒸馏水中,加3滴浓盐酸。试剂1和2在室温下保存稳定,试剂3装入棕色瓶,5～10℃保存,至少可使用2个月。操作步骤:先用吸管吸取1ml尿液于试管中;再加1滴亚硝酸钠溶液和2滴浓盐酸并充分混匀,放置1分钟;最后再加3滴BM溶液,混匀。结果判定:当溶液持续呈紫色时为阳性,如果试验用分光光度计判读,最适宜的波长为540nm。如果溶液不呈紫色或者紫色很快
This article describes an improved method for the determination of sulphonamide Bratton-Marshall. This method is not only easy to get reagents, but also in remote areas with crude equipment can also be used laboratory. Reagents: 1. Sodium nitrite solution (dissolved in distilled water), 0.03 mmol / l or 0.2 mg / 100 ml or 0.2% (W / V). Concentrated hydrochloric acid. 3. Bratton-Marshall solution (BM): 20 mg of N- (1-naphthyl) ethylenediamine dihydrochloride was dissolved in 20 ml of distilled water and 3 drops of concentrated hydrochloric acid were added. Reagents 1 and 2 are stored at room temperature. Reagent 3 is placed in a brown bottle and stored at 5 to 10 ° C for at least 2 months. Procedure: Pipette 1ml of urine in a test tube; add 1 drop of sodium nitrite solution and 2 drops of concentrated hydrochloric acid and mix thoroughly, place for 1 minute; finally add 3 drops of BM solution and mix well. Determination of the results: When the solution was purple when positive, if the test with a spectrophotometer, the most appropriate wavelength of 540nm. If the solution is not purple or purple quickly