论文部分内容阅读
目的了解陕西省定边县鼠疫疫区啮齿动物巴尔通体感染情况及携带种类,为巴尔通体感染的预防控制提供科学依据。方法 2015年在定边县鼠疫疫区的2个乡镇捕获啮齿动物,无菌取鼠脾,分离培养巴尔通体,疑似菌株提取DNA,PCR扩增glt A基因,测序并根据序列应用Mega 6.06软件进行系统发育分析,确定巴尔通体属种。结果共捕获3种223只啮齿动物,分离巴尔通体78株,感染率为34.98%,其中主要啮齿动物长爪沙鼠的感染率为34.72%;序列分析表明定边县鼠疫疫区共检出4个巴尔通体种群:伊丽莎白巴尔通体(B.elizabethae)、格拉汉姆巴尔通体(B.grahamii),另外2个种群与泰勒巴尔通体(B.taylorii)、文森巴尔通体阿鲁潘亚种(B.vinsonii subsp.arupensis)亲缘关系较近,有待进一步确认。结论陕西省定边县鼠疫疫区啮齿动物中有巴尔通体感染,存在人群感染的风险。
Objective To understand the rodent Bartonella infection and the types of plague in the plague area of Dingbian County, Shaanxi Province, and to provide a scientific basis for the prevention and control of Balton infection. Methods Rodents were captured in two townships in the plague-endemic area of Dingbian County in 2015. The spleens were isolated aseptically from Balb / c mice and the DNA was extracted from suspected strains. The glt A gene was amplified by PCR and sequenced. The sequences were sequenced using Mega 6.06 software Phylogenetic analysis to determine Bartonella species. Results A total of 223 223 rodents were isolated and 78 isolates of Barr, the infection rate was 34.98%. The infection rate of the main rodent Mongolian gerbils was 34.72%. Sequence analysis showed that 4 B. Bartonella populations: B. elizabethae, B. grahamii and the other two populations were closely related to B. tylorii, B. vincentis, .vinsonii subsp.arupensis) has a close relationship and remains to be confirmed. Conclusions There is Barr-Bartonella infection in rodents in the plague-endemic area of Dingbian County, Shaanxi Province, and there is a risk of human infection.