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1.大白鼠飼以含p-DAB膳食,第7天即見肝細胞胞浆嗜碱性物貭減少,并出現由嗜酸性細小顆粒聚集而成之“包涵体”状物。随肝細胞的退变及破坏,胆管上皮逐漸增生,至28天、肝小叶边緣大部被新生之胆管細胞所占据。殘存之肝細胞,有少部分呈現异常之肥大。 2.肝細胞在p-DAB作用下,核糖核酸,糖元,蛋白貭結合硫氫基等均見減少,琥珀酸脱氢酶、非特异性酯酶活性减弱,伴以脂肪滴的出现和增多及碱性磷酸酶活性加强。 3.肥大之肝细胞除偶见有“包涵体”状物外,大致呈现正常肝细胞的组织化学反应。 4.肝细胞中出现的嗜酸性“包涵体”状物不被派若宁所着色,PAS反应阳性,硫氢基反应亦较强,碱性磷酸酶反应与胞浆其他成分一致。 5.随胆管上皮之增殖,其核糖核酸,硫氢基,酯酶活性等逐见增加。新生的细胞具有碱性磷酸酶的强阳性反应。 6.以上变化表示致癌物质对肝细胞的破坏作用和肝脏因而引起的反应性和代偿性的变化。
1. Rats were fed a diet containing p-DAB. On the 7th day, the cytoplasmic basophils of hepatocytes were reduced, and “inclusion bodies” formed by aggregation of eosinophilic fine particles appeared. With the degeneration and destruction of hepatocytes, the bile duct epithelium gradually proliferated, and by 28 days, most of the edges of the hepatic lobule were occupied by new born bile duct cells. Some of the remaining hepatocytes exhibit abnormal hypertrophy. 2. Under the action of p-DAB, hepatocytes have reduced ribonucleic acid, glycogen, and peptone-binding sulfhydryl groups, and decreased activity of succinate dehydrogenase and non-specific esterase, accompanied by the appearance and increase of fat droplets. Alkaline phosphatase activity is enhanced. 3. Hypertrophic hepatocytes, in addition to occasional “inclusion body” appearance, roughly presents normal liver cell histochemical reactions. 4. The eosinophilic “inclusion bodies” appearing in hepatocytes are not colored by Pyronin, the PAS reaction is positive, and the sulfhydryl group reaction is also strong. The alkaline phosphatase reaction is consistent with other components of the cytoplasm. 5. With the proliferation of bile duct epithelium, its ribonucleic acid, sulfhydryl groups, esterase activity increased gradually. Neonate cells have a strong positive reaction with alkaline phosphatase. 6. The above changes represent the destructive effect of carcinogens on liver cells and the resulting changes in reactivity and compensation of the liver.