论文部分内容阅读
目的:探讨银杏提取物(GBE)对实验性自身免疫性脑脊髓炎(EAE)小鼠炎症脱髓鞘病变的影响。方法:应用髓鞘少突胶质细胞糖蛋白33-55(MOG33-55)配以完全弗氏佐剂(CFA)免疫小鼠,诱发EAE模型。将小鼠分为CFA对照组、EAE模型组和GBE治疗组(每日腹腔注射GBE70mg/kg)。通过神经功能评分、行为学实验以及免疫荧光染色,观察GBE对EAE小鼠的影响。结果:GBE组小鼠各时间段神经功能评分均低于EAE组(P<0.05),行为学检测显示发病高峰期falling latency时间较EAE组延长10s;GBE组较EAE组视神经髓鞘碱性蛋白(MBP)表达水平增高,可见MBP阳性髓鞘结构包绕轴突;海马伞矢状切片免疫荧光染色证实GBE组CD11b阳性小胶质细胞较EAE组明显减少,但是GFAP阳性星形胶质细胞数量与EAE组无明显差别。结论:GBE可能通过抑制小胶质细胞激活从而延缓EAE小鼠脱髓鞘进程,提示GBE对多发性硬化具有一定的治疗作用。
Objective: To investigate the effect of Ginkgo biloba extract (GBE) on inflammatory demyelination in experimental autoimmune encephalomyelitis (EAE) mice. Methods: EAE model was induced by immunization of mice with myelin oligodendrocyte glycoprotein 33-55 (MOG33-55) and complete Freund’s adjuvant (CFA). The mice were divided into CFA control group, EAE model group and GBE treatment group (daily intraperitoneal injection of GBE70mg / kg). Neurological function score, behavior test and immunofluorescence staining were used to observe the effect of GBE on EAE mice. Results: The scores of neurological function in GBE group were lower than those in EAE group (P <0.05), and the behavioral tests showed that the latency latency of GBE group was 10s longer than that in EAE group. Compared with EAE group, (MBP) expression increased MBP positive myelin sheath wrapped around the axon; hippocampal sagittal sections of the immunofluorescence staining confirmed GBE CD11b-positive microglia significantly reduced compared with EAE group, but the number of GFAP-positive astrocytes No significant difference with EAE group. CONCLUSION: GBE may decelerate the demyelination process of EAE mice by inhibiting the activation of microglia, suggesting that GBE has a certain therapeutic effect on multiple sclerosis.