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目的 :探讨激光诱发自体荧光 (LIF)诊断大肠癌光谱参数的意义。方法 :采用激光诱发自体荧光技术对6 1例大肠癌标本进行了检测。结果 :5 6例 (91.8% )癌标本主峰峰强度 <5 5 0 0U ,次峰峰强度 <2 0 0 0U ,波谷强度<180 0U ;而相应的非癌对照组织主峰峰强度 >110 0 0U ,次峰峰强度 >40 0 0U ,波谷强度 >3 80 0U ;相差分别为1∶3 .36、1∶5 .94、1∶5 .2 2。与非癌组织比较 ,癌组织主峰波长略偏红光 ,次峰略偏紫光 ,主、次峰间的波下降速率相对较缓。用本研究标准判别 ,LIF诊断大肠癌 ,敏感性为 91.8% ,特异性为 10 0 %。结论 :癌组织与非癌组织的LIF有明显差异 ,LIF作为一种无创性内镜下恶性病变的筛检方法 ,用以引导内镜活检有良好的发展前景。
Objective: To investigate the significance of laser-induced autofluorescence (LIF) in the diagnosis of colorectal cancer spectrum parameters. Methods: Totally 61 colorectal cancer specimens were detected by laser-induced autofluorescence. Results: The main peak intensity of 5 6 0 cases (91.8%) was less than 5 5 0 0U, the sub-peak intensity was less than 2 0 0 U and the trough intensity was less than 180 0 U. The corresponding peak intensity of non-cancer control tissue was> 110 0 0U , Sub-peak intensity> 40 0 0U, trough strength> 3 80 0U; the difference was 1: 3.36, 1: 5.94, 1: 5, respectively. Compared with non-cancerous tissue, the main peak wavelength of cancer tissue is slightly reddish, the next peak is slightly violet, and the wave decay rate between the main and the second peak is relatively slow. Using the criteria of this study, the sensitivity and specificity of LIF in the diagnosis of colorectal cancer were 91.8% and 100% respectively. CONCLUSIONS: There are significant differences in LIF between cancerous tissue and non-cancerous tissue. LIF as a noninvasive screening method for endoscopic malignant lesions can guide the development of endoscopic biopsy.