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Reconstruction of human nuclear transfer em-bryos is a necessary step of therapeutic cloning. In this study we injected somatic cell nuclei into MⅡ oocytes and acti-vated reconstructed oocytes with calcium ionophore A23187 (CaA) and 6-dimethylaminopurine (6-DMAP). After oocyte activation and 2PN formation, we removed the female PN. By using this method, we avoided the application of DNA fluorescent stain and ultraviolet light for oocyte enucleation, and over elimination of ooplasm was also mitigated. Some reconstructed embryos developed into the blastocyst stage in vitro.
Reconstruction of human nuclear transfer em-bryos is a necessary step of therapeutic cloning. In this study we injected somatic cell nuclei into MII oocytes and acti-vated reconstructed oocytes with calcium ionophore A23187 (CaA) and 6-dimethylaminopurine (6-DMAP). By using this method, we avoided the application of DNA fluorescent stain and ultraviolet light for oocyte enucleation, and over elimination of ooplasm was also mitigated. Some reconstructed embryos developed into the blastocyst stage in vitro.